Overview of Ribosome Profiling

Translation is known to be important in day-to-day activities. The ribosome and the translation process are closely associated. Because of this, knowing the quantity and location of ribosomes on mRNA in vivo is essential to comprehending how translation is regulated. It's exciting to note that ribosome profiling techniques offer fresh insights into the quantity and locations of ribosomes on mRNAs.

To determine which mRNAs are actively translated, a technique known as ribosome profiling, or Ribo-Seq (ribosome sequencing), mainly uses mRNA sequencing. The technology works on a fairly straightforward principle. Every ribosome encapsulates a brief mRNA segment. Because of ribosome protection, these brief segments of overlaid mRNA are shielded from exogenous RNase digestion. Thus, by creating these fragments and examining their sequence, the location of the ribosome can be ascertained for organisms for which we know the transcriptome sequence. It's important to remember that RNA-Seq sequences every mRNA of a particular sequence found in a sample, whereas Ribo-Seq mainly targets mRNA sequences shielded by ribosomes during translation.