The ready-made mouse brain CD1 cortical neurons were isolated from the mouse CD1 brain (E14, 15). These are all cell suspensions of high-quality primary embryonic brain neuron cells (including glial cells) prepared by standardized methods and can be cultured immediately.
Each vial cortex cell contains approximately 4 million living cells. Using the recommended seeding density and medium, seed it into 10 or more wells of a 24-well plate and 50 or more wells of a 96-well plate. After about 4 days of culture, the cells will form a network of neurites, and by day 7, fragmentation will be minimized.
Recommended medium: CocKit™ Primary Neuron Growth Medium (NCC20-9PZ10)
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